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si appendix  (Thermo Fisher)


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    Thermo Fisher si appendix
    Si Appendix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si+appendix/SUCROSE+EP%2FBP%2FNF+12KG/pm41284850-225-4-14
    Average 99 stars, based on 1 article reviews
    si appendix - by Bioz Stars, 2026-10
    99/100 stars

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    Targeted Proteomics:

    Article Title: Prebiotic organic compounds in samples of asteroid Bennu indicate heterogeneous aqueous alteration.
    Article Snippet: .. MRM transitions listed in SI Appendix, Table S2 were determined and optimized using the ThermoFisher AutoSRM software package. ..

    Software:

    Article Title: Prebiotic organic compounds in samples of asteroid Bennu indicate heterogeneous aqueous alteration.
    Article Snippet: .. MRM transitions listed in SI Appendix, Table S2 were determined and optimized using the ThermoFisher AutoSRM software package. ..

    Construct:

    Article Title: Systematic Discovery of Novel Phosphoinositide-Binding Effectors in Legionella Reveals Conserved α-Helical Folds
    Article Snippet: Protein binding to lipids was visualized with an anti-GST-HRP conjugated antibody (1:2,000) using a ChemiDoc Touch Imaging system (Bio-Rad). .. HeLa cells were transiently co-transfected for 16-20 h with mCherry and GFP-tagged constructs as listed in SI Appendix, Table S2 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific). .. Cells were fixed in PBS with 4% paraformaldehyde for 20 min at room temperature, and coverslips were mounted using ProLong diamond anti-fade mountant (ThermoFisher Scientific).

    Transfection:

    Article Title: Systematic Discovery of Novel Phosphoinositide-Binding Effectors in Legionella Reveals Conserved α-Helical Folds
    Article Snippet: Protein binding to lipids was visualized with an anti-GST-HRP conjugated antibody (1:2,000) using a ChemiDoc Touch Imaging system (Bio-Rad). .. HeLa cells were transiently co-transfected for 16-20 h with mCherry and GFP-tagged constructs as listed in SI Appendix, Table S2 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific). .. Cells were fixed in PBS with 4% paraformaldehyde for 20 min at room temperature, and coverslips were mounted using ProLong diamond anti-fade mountant (ThermoFisher Scientific).

    Gene Knockout:

    Article Title: Identification of claudin-3 as an entry factor for rat hepacivirus.
    Article Snippet: .. Gene knockout was mediated by CRISPR- Cas9 system and the oligos of sgRNA are listed in SI Appendix, Table S2. qRT- PCR was carried out using Fast SYBR Green Master Mix (Applied Biosystems, Waltham, MA) and a QuantStudio 3 Real- Time PCR System (Applied Biosystems). ..

    Article Title: Identification of claudin-3 as an entry factor for rat hepacivirus
    Article Snippet: .. Gene knockout was mediated by CRISPR-Cas9 system and the oligos of sgRNA are listed in SI Appendix , Table S2 . qRT-PCR was carried out using Fast SYBR Green Master Mix (Applied Biosystems, Waltham, MA) and a QuantStudio 3 Real-Time PCR System (Applied Biosystems). ..

    CRISPR:

    Article Title: Identification of claudin-3 as an entry factor for rat hepacivirus.
    Article Snippet: .. Gene knockout was mediated by CRISPR- Cas9 system and the oligos of sgRNA are listed in SI Appendix, Table S2. qRT- PCR was carried out using Fast SYBR Green Master Mix (Applied Biosystems, Waltham, MA) and a QuantStudio 3 Real- Time PCR System (Applied Biosystems). ..

    Article Title: Identification of claudin-3 as an entry factor for rat hepacivirus
    Article Snippet: .. Gene knockout was mediated by CRISPR-Cas9 system and the oligos of sgRNA are listed in SI Appendix , Table S2 . qRT-PCR was carried out using Fast SYBR Green Master Mix (Applied Biosystems, Waltham, MA) and a QuantStudio 3 Real-Time PCR System (Applied Biosystems). ..

    Quantitative RT-PCR:

    Article Title: Identification of claudin-3 as an entry factor for rat hepacivirus.
    Article Snippet: .. Gene knockout was mediated by CRISPR- Cas9 system and the oligos of sgRNA are listed in SI Appendix, Table S2. qRT- PCR was carried out using Fast SYBR Green Master Mix (Applied Biosystems, Waltham, MA) and a QuantStudio 3 Real- Time PCR System (Applied Biosystems). ..

    Article Title: Identification of claudin-3 as an entry factor for rat hepacivirus
    Article Snippet: .. Gene knockout was mediated by CRISPR-Cas9 system and the oligos of sgRNA are listed in SI Appendix , Table S2 . qRT-PCR was carried out using Fast SYBR Green Master Mix (Applied Biosystems, Waltham, MA) and a QuantStudio 3 Real-Time PCR System (Applied Biosystems). ..

    Article Title: Fertile androgenetic mice generated by targeted epigenetic editing of imprinting control regions.
    Article Snippet: The RNA was then used as a template for cDNA synthesis with SuperScript III first- strand synthesis reagents (Invitrogen) with random hexamers. .. The mRNA quantity was determined by quantitative RT–PCR on an Applied Biosystems 7500 system using the primer sequences shown in SI Appendix, Table S6 and SYBR Green SuperMix UDG (Invitrogen). .. PCR was performed in a final volume of 25 μl, consisting of a diluted cDNA sample, 1 × SYBR Green Mix (Invitrogen), primers optimized for each target gene, and nuclease- free water.

    SYBR Green Assay:

    Article Title: Identification of claudin-3 as an entry factor for rat hepacivirus.
    Article Snippet: .. Gene knockout was mediated by CRISPR- Cas9 system and the oligos of sgRNA are listed in SI Appendix, Table S2. qRT- PCR was carried out using Fast SYBR Green Master Mix (Applied Biosystems, Waltham, MA) and a QuantStudio 3 Real- Time PCR System (Applied Biosystems). ..

    Article Title: Identification of claudin-3 as an entry factor for rat hepacivirus
    Article Snippet: .. Gene knockout was mediated by CRISPR-Cas9 system and the oligos of sgRNA are listed in SI Appendix , Table S2 . qRT-PCR was carried out using Fast SYBR Green Master Mix (Applied Biosystems, Waltham, MA) and a QuantStudio 3 Real-Time PCR System (Applied Biosystems). ..

    Article Title: Fertile androgenetic mice generated by targeted epigenetic editing of imprinting control regions.
    Article Snippet: The RNA was then used as a template for cDNA synthesis with SuperScript III first- strand synthesis reagents (Invitrogen) with random hexamers. .. The mRNA quantity was determined by quantitative RT–PCR on an Applied Biosystems 7500 system using the primer sequences shown in SI Appendix, Table S6 and SYBR Green SuperMix UDG (Invitrogen). .. PCR was performed in a final volume of 25 μl, consisting of a diluted cDNA sample, 1 × SYBR Green Mix (Invitrogen), primers optimized for each target gene, and nuclease- free water.

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of claudin-3 as an entry factor for rat hepacivirus.
    Article Snippet: .. Gene knockout was mediated by CRISPR- Cas9 system and the oligos of sgRNA are listed in SI Appendix, Table S2. qRT- PCR was carried out using Fast SYBR Green Master Mix (Applied Biosystems, Waltham, MA) and a QuantStudio 3 Real- Time PCR System (Applied Biosystems). ..

    Article Title: Identification of claudin-3 as an entry factor for rat hepacivirus
    Article Snippet: .. Gene knockout was mediated by CRISPR-Cas9 system and the oligos of sgRNA are listed in SI Appendix , Table S2 . qRT-PCR was carried out using Fast SYBR Green Master Mix (Applied Biosystems, Waltham, MA) and a QuantStudio 3 Real-Time PCR System (Applied Biosystems). ..

    Isolation:

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus .
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn- Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. 48) with the following modifications: 1) MycoMar specific primers were used (SI Appendix, Table S5), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR- 1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR- 2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR- 2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn-Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. ) with the following modifications: 1) MycoMar specific primers were used ( SI Appendix , Table S5 ), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR-1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR-2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR-2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Sequencing:

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus .
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn- Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. 48) with the following modifications: 1) MycoMar specific primers were used (SI Appendix, Table S5), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR- 1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR- 2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR- 2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn-Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. ) with the following modifications: 1) MycoMar specific primers were used ( SI Appendix , Table S5 ), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR-1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR-2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR-2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Mutagenesis:

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus .
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn- Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. 48) with the following modifications: 1) MycoMar specific primers were used (SI Appendix, Table S5), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR- 1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR- 2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR- 2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn-Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. ) with the following modifications: 1) MycoMar specific primers were used ( SI Appendix , Table S5 ), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR-1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR-2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR-2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Purification:

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus .
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn- Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. 48) with the following modifications: 1) MycoMar specific primers were used (SI Appendix, Table S5), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR- 1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR- 2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR- 2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn-Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. ) with the following modifications: 1) MycoMar specific primers were used ( SI Appendix , Table S5 ), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR-1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR-2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR-2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Article Title: Supplementary Information for Retrotransposon-mediated evolutionary rewiring of a pathogen response orchestrates a resistance phenotype in an insect host
    Article Snippet: Briefly, two single-guide RNA (sgRNA1 and sgRNA2) were designed using the CRISPR RGEN tool Cas-Designer (http://www.rgenome.net/cas-designer/). .. The DNA template of sgRNAs was synthesized as listed in SI Appendix, Table S5, and the high yield sgRNAs were produced by the MEGAshortscript Transcription Kit (Ambion) and further purified by the MEGAclear Kit (Ambion). ..

    Polymerase Chain Reaction:

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus .
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn- Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. 48) with the following modifications: 1) MycoMar specific primers were used (SI Appendix, Table S5), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR- 1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR- 2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR- 2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn-Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. ) with the following modifications: 1) MycoMar specific primers were used ( SI Appendix , Table S5 ), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR-1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR-2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR-2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Size Selection:

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus .
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn- Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. 48) with the following modifications: 1) MycoMar specific primers were used (SI Appendix, Table S5), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR- 1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR- 2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR- 2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn-Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. ) with the following modifications: 1) MycoMar specific primers were used ( SI Appendix , Table S5 ), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR-1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR-2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR-2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Multiplex Assay:

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus .
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn- Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. 48) with the following modifications: 1) MycoMar specific primers were used (SI Appendix, Table S5), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR- 1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR- 2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR- 2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn-Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. ) with the following modifications: 1) MycoMar specific primers were used ( SI Appendix , Table S5 ), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR-1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR-2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR-2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Sample Prep:

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus .
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn- Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. 48) with the following modifications: 1) MycoMar specific primers were used (SI Appendix, Table S5), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR- 1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR- 2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR- 2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Article Title: The role of colony morphotype in shaping gene essentiality in Mycobacteroides abscessus
    Article Snippet: .. Isolated DNA was prepared for transposon sequencing following a previously published protocol (see Tn-Seq Illumina Library Preparation from Initial PA14 Mutant Library in supplemental material for ref. ) with the following modifications: 1) MycoMar specific primers were used ( SI Appendix , Table S5 ), 2) between steps, DNA was purified using the GeneJet PCR purification kit (Thermo Scientific, Waltham, MA); 3) 2 to 4 PCR-1 reactions were performed, each with 0.5 to 1 μg DNA and pooled in the following purification step; 4) PCR-2 was carried out with a mixture of 4 primers containing 3, 5, 7, or 9 random bases to increase the sequence diversity of our libraries; and 5) DNA size selection after PCR-2 was performed as described in the NEB “QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina” protocol. ..

    Synthesized:

    Article Title: Supplementary Information for Retrotransposon-mediated evolutionary rewiring of a pathogen response orchestrates a resistance phenotype in an insect host
    Article Snippet: Briefly, two single-guide RNA (sgRNA1 and sgRNA2) were designed using the CRISPR RGEN tool Cas-Designer (http://www.rgenome.net/cas-designer/). .. The DNA template of sgRNAs was synthesized as listed in SI Appendix, Table S5, and the high yield sgRNAs were produced by the MEGAshortscript Transcription Kit (Ambion) and further purified by the MEGAclear Kit (Ambion). ..

    Produced:

    Article Title: Supplementary Information for Retrotransposon-mediated evolutionary rewiring of a pathogen response orchestrates a resistance phenotype in an insect host
    Article Snippet: Briefly, two single-guide RNA (sgRNA1 and sgRNA2) were designed using the CRISPR RGEN tool Cas-Designer (http://www.rgenome.net/cas-designer/). .. The DNA template of sgRNAs was synthesized as listed in SI Appendix, Table S5, and the high yield sgRNAs were produced by the MEGAshortscript Transcription Kit (Ambion) and further purified by the MEGAclear Kit (Ambion). ..



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